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B 710/50. BD LSRFortessa ™ X-20 cell analyzer Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. Performance without peer, choice without compromise. The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

The X-20 is equipped with 4 different lasers. It can detect up to 16 colors simultaneously. Instead of calling the detection channels generically Mar 10, 2017 Three flow cytometers (BD FACS Canto™ II, BD LSR Fortessa™ and MACS and eight fluorescence emission channels (FL1-FL8) were used. 2014 BD. The BD Horizon™ Tour: New insights for multicolor panel design /2. Revealing What is other channels: Directly and irreversibly reduces the resolution sensitivity of that channel; Contributes to background BD LSR Fortessa Mid Bead Median. Channel. MFI value of mid beads.

A term used in the calculation of photon detection efficiency. (Qr) and linearity. Mid Bead % Robust CV. BD Aria III. 4 lasers (488nm, 405nm, 561nm, 640nm) 16 fluorescent colours, capable of The Aurora has 5 lasers, 3 scatter channels and 64 fluorescence channels.

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Revealing What is other channels: Directly and irreversibly reduces the resolution sensitivity of that channel; Contributes to background BD LSR Fortessa Mid Bead Median. Channel. MFI value of mid beads.

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4. Remove the sheath container lid. 5. The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser.

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Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm). BD Fortessa X20 A Configuration and Fluorochromes Laser Diva Parameter Name Fluorochromes 488 nm Blue B 530/30 BB515, FITC, AF488, CFSE, GFP, TO-PRO-1 B 695/40 BB700, PerCP, PerCP-Cy5.5, PerCP-eFluor710, 7-AAD, PI 633 nm Red R 670/30 APC, AF633, AF647, TO-PRO-3 R 730/45 AF 700, APC-Cy5.5, Dye Cycle Ruby R 780/60 APC-Cy7, APC-H7, APC-eFluor780 BD LSR Fortessa SORP – How to Use Guide 7 26 In the Experiment menu select “Experiment Layout” and write the name of the molecules you are analyzing in the corresponding fluorochrome/channel. 27 In the Acquisition Dashboard panel select the number of events to visualize and The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336, VAT Registration Number GB 592 9507 00, and is acknowledged by the UK authorities as a “Recognised body” which has been granted degree awarding powers. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig.

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